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Perbandingan Optimasi Biosensor Antioksidan Menggunakan Ekstrak dan Enzim Murni Superoksida Dismutase

Abstract: An antioxidant biosensor has been developed for measurement of antioxidant capacity. The purpose of this study was to determine and compare the activity of the superoxide dismutase (SOD) enzyme from the crude extract of Deinococcus radiodurans cells and pure SOD from bovine erythrocytes which were immobilized on zeolite nanoparticles (ZNP).
The kinetics parameters were also determined. The optimum condition was obtained by Response Surface Method. The immobilized crude extract D. radiodurans SOD (CES) modified carbon paste electrode (CPE) (CES-ZNP-CPE) have different optimum conditions with the modified carbon paste electrode pure SOD immobilization (PS-ZNP-CPE). This is shown by the difference in the two structures. Erythrocyte SOD enzyme from cows that have the type of Cu / Zn-SOD, whereas SOD extracted from the bacterium D. radiodurans is the type of Mn-SOD. The differences in the structure cause the Cu / Zn SOD and Mn-SOD has a different reaction mechanism, so that responses and measurement will be differences resulted.

Keywords: Antioxidant biosensor, Immobilization, Superoxide dismutase, Zeolite nanoparticles.

Imas Eva Wijayanti

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